In vitro Antitrypanosomal, Antioxidant and Cytotoxicity Activities, LC MS analysis and Molecular docking analysis of bioactive compounds from Anopyxis klaineana against UDP Galactose 4` Epimerase (GalE) of Trypanosoma brucei

Abstract

African Trypanosomiasis is a major public health concern worldwide, especially in developing countries 1 Current chemotherapies are highly toxic, resistant and ineffective Hence, novel effective and potent trypanocides are needed Medicinal plants have been documented to be a potential source for the development of antitrypanosomal compounds Anopyxis klaineana is an ethnomedicinal plant used in west Africa to treat many ailments including protozoan diseases In this study, we investigated the in vitro effects of crude methanol extracts and fractions of A klaineana for their antitrypanosomal activities against Trypanosoma brucei using Alamar blue assay Additionally, the crude extract’s antioxidant and cytotoxicity activities were also determined The phytochemical profiling of the crude extract was determined using LC ESI QTOF MS to identify major bioactive compounds present Bioactive compounds identified were subjected to molecular docking studies against Trypanosoma brucei’s UDP Galactose 4 Epimerase (TbGalE